Plug-and-play assay portal

Build your own targeted phosphoproteomic assay!

	Select phosphosites (manual)
	Select phosphosites (automatic)
	Browse pre-built assays
	Upload a list of sites

Protein:

Protein reference

Uniprot ID

Site:

Select all positional isomers

Digestion enzyme:

LysC/Trypsin

Trypsin

Select peptide sequences

No records found.

Input measured retention times for calibration. (default: 60 minute uncalibrated method)

Calibrate retention time

Fill the retention time column using your run information.

SSAAPPPPPR SSAAPPPPPR		8.31
GISNEGQNASIK GISNEGQNASIK		9.19
HVLTSIGEK HVLTSIGEK		11.13
DIPVPKPK DIPVPKPK		14.72
IGDYAGIK IGDYAGIK		15.04
TASEFDSAIAQDK TASEFDSAIAQDK		18.31
SAAGAFGPELSR SAAGAFGPELSR		20.91
ELGQSGVDTYLQTK ELGQSGVDTYLQTK		23.48
GLILVGGYGTR GLILVGGYGTR		29.66
SFANQPLEVVYSK SFANQPLEVVYSK		31.5
LTILEELR LTILEELR		34.51
NGFILDGFPR NGFILDGFPR		37.28
ELASGLSFPVGFK ELASGLSFPVGFK		39.5

Run time (min):

Select desired retention time scheduling window. (default +/- 5 minutes)

Retention time (+/- min):

No peptides added

This file can be imported into the mass spectrometer method as an inclusion list.

Schedule optimization:

Select a protein

Choose a protein in order to select phosphorylation sites.

Reference	Uniprot ID	Description
1433B	P31946-2	Isoform Short of 14-3-3 protein beta/alpha
2A5G	Q13362-4	Isoform 4 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
2ABB	Q00005-6	Isoform 6 of Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform
41	P11171-6	Isoform 6 of Protein 4.1
44M2.3	Q96IC2	Putative RNA exonuclease NEF-sp
4F2	P08195-2	Isoform 2 of 4F2 cell-surface antigen heavy chain
5HT3D	Q70Z44-4	Isoform 4 of 5-hydroxytryptamine receptor 3D
5HT4R	Q13639-4	Isoform 5-HT4(D) of 5-hydroxytryptamine receptor 4
5NT3A	Q9H0P0-1	Isoform 1 of Cytosolic 5'-nucleotidase 3A
8ODP	P36639-2	Isoform p22 of 7,8-dihydro-8-oxoguanine triphosphatase

Step 1: Select sites