Plug-and-play assay portal

Build your own targeted phosphoproteomic assay!

Step 1: Select sites

    Select peptide sequences

    SequenceBest AscoreiRTCountEnrichment methodDigestion enzyme
    No records found.

    Step 2: Calibrate retention time

    Input measured retention times for calibration. (default: 60 minute uncalibrated method)

    Calibrate retention time

    Step 3: Refine assay

    Select desired retention time scheduling window. (default +/- 5 minutes)

    Retention time +/- RT tolerance

    Current Assay

    Mass [m/z]CS [z]Start [min]End [min]Comment
    No peptides added

    Step 4: Export schedule

    This file can be imported into the mass spectrometer method as an inclusion list.

    Schedule optimization:

    Select a protein

    Choose a protein in order to select phosphorylation sites.

    ReferenceUniprot IDDescription
    1433BP31946-2Isoform Short of 14-3-3 protein beta/alpha
    2A5GQ13362-4Isoform 4 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
    2ABBQ00005-6Isoform 6 of Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform
    41P11171-6Isoform 6 of Protein 4.1
    44M2.3Q96IC2Putative RNA exonuclease NEF-sp
    4F2P08195-2Isoform 2 of 4F2 cell-surface antigen heavy chain
    5HT3DQ70Z44-4Isoform 4 of 5-hydroxytryptamine receptor 3D
    5HT4RQ13639-4Isoform 5-HT4(D) of 5-hydroxytryptamine receptor 4
    5NT3AQ9H0P0-1Isoform 1 of Cytosolic 5'-nucleotidase 3A
    8ODPP36639-2Isoform p22 of 7,8-dihydro-8-oxoguanine triphosphatase
    Upload Calibration Data
    Upload Schedule
    Upload a list of sites
    Upload a CSV file containing the header: Protein,Site.
    Select the protein identification:
    Site format: Aminoacid and Position (ex: S125)