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Plug-and-play assay portal 2.0

Welcome to Phosphopedia's new assay portal interface. Here you will be able to access the updated database, for multiple organisms, through a single standardized form.

Step 1: Select sites

Select which database to pull information from. (Coming Soon: mixed assays)

Human
Yeast
FASTA
UniProt Entry
UniProt ID

Select peptide sequences

SequenceModificationsRetention Time
SitesAscoresiRT#ExamplesError
No records found.




Select a target

SequenceChargeM/ZSpectral CountiRT
No records found.




Step 2: Calibrate retention time

Input measured retention times for calibration. (Default: 60 minute uncalibrated method)

Calibrate retention time

Step 3: Refine assay

Select desired retention time scheduling window. (default +/- 5 minutes)

Retention time +/- RT tolerance


Current Assay

Mass [m/z]CS [z]Start [min]End [min]Comment
No peptides added

Step 4: Export schedule

This file can be imported into the mass spectrometer method as an inclusion list.

If you use this resource, please cite: Robert T. Lawrence, Brian C. Searle, Ariadna Llovet, Judit Villén. Plug-and-play analysis of the human phosphoproteome by targeted high-resolution mass spectrometry. Nature Methods. 2016.

Upload Calibration Data
Choose